Duration: 1-2 years
Project description
- To functionally characterize novel mutations identified in a panel of neurodegenerative diseases, ranging from frontotemporal dementia (FTD), and spastic paraplegia (SPG), to amyotrophic lateral sclerosis (ALS).
- These studies will involve developing in vitro (cell lines and primary neuronal cultures) as well as in vivo (zebrafish) models. Both overexpression of these mutant genes and/or knockdown/knockout of the genes will be performed in these models. To study degeneration of subset of neurons in the brain and spinal cord, an array of molecular biology techniques will be used.
- Techniques used:
- Molecular genetics: Latest human and animal molecular genetic techniques
- Molecular biology: DNA cloning, PCR, site directed mutagenesis, in situ hybridization.
- Biochemistry: protein purification, immunoprecipitation, pull-down and Western blotting.
- Zebrafish genetics: Microinjection of RNA and antisense morpholino oligonucleotides. Generation and maintenance of transgenic zebrafish lines.
- Immunohistochemistry and live imaging of zebrafish embryos. Behavioral assays and electrophysiological recordings
Research experience:
- PhD in life or health sciences. Experience in molecular genetics a must.
- Candidates with extensive experience on disease-related animal models are encouraged to apply.
- Strong background in cellular and molecular biology, genetics, and/ biochemistry.
- Motivated and enthusiastic candidate
- Willing to learn and establish novel techniques in the lab
- Good team spirit and work well in collaboration
In order to apply please send your CV, two references and a letter of recommendation to:
Dr. Edor Kabashi: edor.kabashi@icm-institute.org"