Under the general direction of the principal investigator, plan and carry out experiments designed to identify and characterize pathways and processes in sulfate reducing bacteria (SRB) which are targeted by environmentally relevant stresses. Specific attention will be focused on understanding the range of factors responsible for iron sulfur cluster cofactor biosynthesis and repair in SRBs.
SPECIFIC RESPONSIBILITIES:
- Characterize strains of Desulfovibrio vulgaris Hildenborough which possess null mutations in predicted FeS cluster biosynthesis components using biochemical assays for FeS enzyme activities and phenotypic screens.
- Perform chemogenomic fitness profiling of D. vulgaris mutants, using appropriate stress conditions, and contribute to the development of genetic interaction screening techniques which utilize a next generation sequencing based assay.
- Identify and characterize additional genes associated with FeS cluster biosynthesis and other systems linked to energy generation and metal homeostasis using chemogeneomic and genetic interaction fitness profiling techniques. Communicate with informatics analysis and data management colleagues to ensure correct and efficient data integration and interpretation.
- Use advanced spectroscopic approaches (CD, EPR) to characterize proteins of interest where required.
- Provide research support and assistance to other members of the laboratory as requested.
- Keep detailed records of experimental procedures and results and communicate data to group and PI via individual and group meetings.
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